Evaluating methyl jasmonate for induction of resistance to Fusarium oxysporum, F. circinatum and Ophiostoma novo-ulmi
AbstractDamping off is probably the most common disease affecting seedlings in forest nurseries. In south-western Europe, the pitch canker and the Dutch elm disease cause relevant economic looses in forests, mostly in adult trees. The ability of the chemical plant elicitor methyl jasmonate (MeJA) to induce resistance in Pinus pinaster against Fusarium oxysporum and F. circinatum, and in Ulmus minor against Ophiostoma novo-ulmi was examined. In a first experiment, an aqueous solution of MeJA 5 mM was applied to P. pinaster seeds by immersion or spray, and different concentrations of MeJA (0, 0.1, 0.5, 1, 5 and 10 mM) were tested in seedlings before inoculations with F. oxysporum (105 and 107 spores mL–1). In a second experiment, 6-months-old P. pinaster seedlings were sprayed with 0 and 25 mM of MeJA, and later challenged with mycelium of F. circinatum. Finally, 4-year-old U. minor trees were sprayed with 0, 50 and 100 mM of MeJA and subsequently inoculated with O. novo-ulmi (106 spores mL–1). MeJA did not protect P. pinaster seeds and seedlings against F. oxysporum, probably because plants were too young for the physiological mechanisms responsible for resistance to be induced. Based on the morphological changes observed in the treated 6-months-old P. pinaster seedlings (reduction of growth and increased resin duct density), there is evidence that MeJA could have activated the mechanisms of resistance. However, 25 mM MeJA did not reduce plant mortality, probably because the spread of the virulent F. circinatum strain within the tree tissues was faster than the formation of effective defense responses. Based on the lack of phenological changes observed in the treated elms, there is no evidence that MeJA would cause induction of resistance. These results suggest that the use of MeJA to prevent F. oxysporum and F. circinatum in P. pinaster seedlings in nurseries and O. novo-ulmi in U. minor trees should be discarded.
Aegerter BJ, Gordon TR. 2006. Rates of pitch canker induced seedling mortality among Pinus radiata families varying in levels of genetic resistance to Gibberella circinata (anamorph Fusarium circinatum). For Ecol Manage 235, 14-17.
Ali Z, Smith I, Guest DI. 2000. Combinations of potassium phosphonate and Bion (acibenzolar-S-methyl) reduce root infection and dieback of Pinus radiata, Banksia integrifolia and Isopogon cuneatus caused by Phytophthora cinnamomi. Australasian Plant Pathol 29, 59-63.
Alves-Santos FM, Martínez-Bermejo D, Rodríguez-Molina MC, Díez JJ. 2007. Cultural characteristics, pathogenicity and genetic diversity of Fusarium oxysporum isolates from tobacco fields in Spain. Physiol Mol Plant Pathol 71, 26-32. http://dx.doi.org/10.1016/j.pmpp.2007.09.007
Barrows-Broaddus JB, Dwinell LD. 1984. Variation in susceptibility to the pitch canker fungus among half-sib and full-fib families of Virginia pine. Phytopathology 74, 438-444. http://dx.doi.org/10.1094/Phyto-74-438
Bonello P, Gordon TR, Herms DA, Wood DL, Erbiligin N. 2006. Nature and ecological implications of pathogeninduced systemic resistance in conifers: A novel hypothesis. Physiol Mol Plant Pathol 68, 95-104. http://dx.doi.org/10.1016/j.pmpp.2006.12.002
Brener WD, Beckman CH. 1968. A mechanism of enhanced resistance to Ceratocystis ulmi in American elms treated with sodium trichloro-phenylacetate. Phytopathology 58, 555-561.
Correll JC, Gordon TR, McCain AH, Fox JW, Koehler CS, Wood DL, Schultz ME. 1991. Pitch canker disease in California: Pathogenicity, distribution, and canker development on Monterey pine (Pinus radiata). Plant Dis 75, 676-682. http://dx.doi.org/10.1094/PD-75-0676
Davis JM, Wu H, Cooke JEK, Reed JM, Luce KS, Michler CH. 2002. Pathogen challenge, salicyclic acid, and jasmonic acid regulate expression of chitinase gene homologs in pine. Mol Plant-Microbe Interact 15, 380-387. http://dx.doi.org/10.1094/MPMI.2002.15.4.380 PMid:12026177
Develey-Rivière MP, Galiana E. 2007. Resistance to pathogens and host developmental stage: a multifaceted relationship within the plant kingdom. New Phytol 175, 405-416. http://dx.doi.org/10.1111/j.1469-8137.2007.02130.x PMid:17635216
Eyles A, Bonello P, Ganley R, Mohammed C. 2010. Induced resistance to pests and pathogens in trees. New Phytol 185, 893-908. http://dx.doi.org/10.1111/j.1469-8137.2009.03127.x PMid:20015067
Glazebrook J. 2005. Contrasting mechanisms of defense against biotrophic and necrotrophic pathogens. Annual Rev Phytopathology 43, 205-227. http://dx.doi.org/10.1146/annurev.phyto.43.040204.135923 PMid:16078883
Gordon TR, Kirkpatrick SC, Aegerter BJ, Fisher AJ, Storer AJ, Wood DL. 2010. Evidence for the occurrence of induced resistance to pitch canker, caused by Gibberella circinata (anamorph Fusarium circinatum), in populations of Pinus radiata. Forest Pathol 41, 227-232. http://dx.doi.org/10.1111/j.1439-0329.2010.00678.x
Heijari J, Nerg A-M, Kainulainen P, Viiri H, Vuorinen M, Holopainen JK. 2005. Application of methyl jasmonate reduces growth but increases chemical defence and resistance against Hylobius abietis in Scots pine seedlings. Entomol Exp Appl 115, 117-124. http://dx.doi.org/10.1111/j.1570-7458.2005.00263.x
Holopainen JK, Heijari J, Nerg A-M, Vuorinen M, Kainulainen P. 2009. Potential for the use of exogenous chemical elicitors in disease and insect pest management of conifer seedling production. Open For Sci J 2, 17-24.
Huber DPW, Philippe RN, Madilao LL, Sturrock RN, Bohlmann J. 2005. Changes in anatomy and terpene chemistry in roots of Douglas-fir seedlings following treatment with methyl jasmonate. Tree Physiol 25, 1075-1083. http://dx.doi.org/10.1093/treephys/25.8.1075 PMid:15929938
Hudgins JW, Christiansen E, Franceschi VR. 2004. Induction of anatomically based defense response in stems of diverse conifers by methyl jasmonate: a phylogenetic perspective. Tree Physiol 24, 251-264. http://dx.doi.org/10.1093/treephys/24.3.251 PMid:14704135
Kim KW, Lee IJ, Kim CS, Eom IY, Choi JW, Lee DK, Park EW. 2010. Resin flow, symptom development, and lignin biosynthesis of two pine species in response to wounding and inoculation with Fusarium circinatum. Plant Pathol J 26, 394-401. http://dx.doi.org/10.5423/PPJ.2010.26.4.394
Komada H. 1975. Development of a selective medium for quantitative isolation of Fusarium oxysporum from natural soil. Review Plant Prot Research 8, 114–125.
Kozlowski G, Buchala A, Métraux JP. 1999. Methyl jasmonate protects Norway spruce (Picea abies (L.) Karst.) seedlings against Pythium ultimum Trow. Physiol Mol Plant Pathol 55, 53-58. http://dx.doi.org/10.1006/pmpp.1999.0205
Krokene P, Nagy NE, Solheim H. 2008. Methyl jasmonate and oxalic acid treatment of Norway spruce: anatomically based defense responses and increased resistance against fungal infection. Tree Physiol 28, 29-35. http://dx.doi.org/10.1093/treephys/28.1.29 PMid:17938111
Kuhlman EG, Dianis SD, Smith TK. 1982. Epidemiology of pitch canker disease in a loblolly pine seed orchard. Phytopathology 72, 1212-1216. http://dx.doi.org/10.1094/Phyto-72-1212
Kusumoto D, Goldwasser Y, Xie X, Yoneyama K, Takeuchi Y, Yoneyama K. 2007. Resistance of red clover (Trifolium pratense) to the root parasitic plant Orobanche minor is activated by salicylate but not by jasmonate. Annals Bot 100, 537-544. http://dx.doi.org/10.1093/aob/mcm148 PMid:17660517 PMCid:2533599
Machón P, Santamaría O, Pajares JA, Alves-Santos FM, Díez JJ. 2006. Influence of the ectomycorrhizal fungus Laccaria laccata on pre-emergence, post-emergence and late damping-off by Fusarium moniliforme and F. oxysporum on Scots pine seedlings. Symbiosis 42, 153-160.
Martin D, Tholl D, Gershenzon J, Bohlmann J. 2002. Methyl jasmonate induces traumatic resin ducts, terpenoid resin biosynthesis, and terpenoid accumulation in developing xylem of Norway spruce stems. Plant Physiol 129, 1003-1018. http://dx.doi.org/10.1104/pp.011001 PMid:12114556 PMCid:166496
Martín JA, Solla A, Coimbra MA, Gil L. 2008a. Metabolic fingerprinting allows discrimination between Ulmus pumila and U. minor and between U. minor clones of different susceptibility to Dutch elm disease. For Pathol 38, 244-256.
Martín JA, Solla A, Domingues MR, Coimbra MA, Gil L, 2008b. Exogenous phenol increase resistance of Ulmus minor to Dutch elm disease through formation of suberinlike compounds on xylem tissues. Environ Exp Bot 64, 97-104. http://dx.doi.org/10.1016/j.envexpbot.2008.05.004
Martín JA, Solla A, Witzell J, Gil L, García-Vallejo MC. 2010. Antifungal effect and reduction of Ulmus minor symptoms to Ophiostoma novo-ulmi by carvacrol and salicylic acid. Eur J Plant Pathol 127, 21-32. http://dx.doi.org/10.1007/s10658-009-9567-3
McComb JA, O'Brien P, Calver M, Staskowski P, Jardine N, Eshraghi L, Ellery J, Gilovitz J, Scott P, O'Brien J, O'Gara E, Howard K, Dell B, Hardy GEStJ. 2008. Research into natural and induced resistance in Australian native vegetation of Phytophthora cinnamomi and innovative methods to contain and/or eradicate within localized incursions in areas of high biodiversity in Australia. Enhancing the efficacy of phosphite with the addition/supplementation of other chemicals such as those known to be involved in resistance. Report prepared by the Centre for Phytophthora Science and Management for the Australian Government Department of the Environment, Water, Heritage and the Arts. 92 pp.
Moreira X, Sampedro L, Zas R, Solla A. 2008. Alterations of the resin canal system of Pinus pinaster in a healthy and a Hylobious abietis attacked stands. Trees 22, 771-777. http://dx.doi.org/10.1007/s00468-008-0237-4
Moreira X, Sampedro L, Zas R. 2009. Defensive responses of Pinus pinaster seedlings to exogenous application of methyl jasmonate: Concentration effect and systemic response. Environ Exp Bot 67, 94-100. http://dx.doi.org/10.1016/j.envexpbot.2009.05.015
Panter SN, Jones DA. 2002. Age-related resistance to plant pathogens. Adv Bot Res 38, 251-280. http://dx.doi.org/10.1016/S0065-2296(02)38032-7
Phillips MA, Croteau RB. 1999. Resin-based defenses in conifers. Trends Plant Sci 4, 184-190. http://dx.doi.org/10.1016/S1360-1385(99)01401-6
Pieterse CMJ, Van Loon LC. 2007. Signalling cascades involved in induced resistance. In: Induced resistance for plant defence: a sustainable approach to crop protection (Walters D., ed). Blackwell plublishing, Oxford, UK, pp. 65-88.
Reglinski T, Stavely FJL, Taylor JT. 1998. Induction of phenylalanine ammonia lyase activity and control of Sphaeropsis sapinea infection in Pinus radiata by 5-chlorosalicylic acid. Eur J For Pathol 28, 153–158. http://dx.doi.org/10.1111/j.1439-0329.1998.tb01245.x
Scheffer RJ, Voeten JGWF, Guries RP. 2008. Biological control of Dutch elm disease. Plant Dis 92, 192-200. http://dx.doi.org/10.1094/PDIS-92-2-0192
Solla A, Gil L. 2003. Evaluating Verticillium dahliae for biological control of Ophiostoma novo-ulmi in Ulmus minor. Plant Pathol 52, 579-585. http://dx.doi.org/10.1046/j.1365-3059.2003.00921.x
Solla A, Martín JA, Ouellette GB, Gil L. 2005. Influence of plant age on symptom development in Ulmus minor following inoculation by Ophiostoma novo-ulmi. Plant Dis 89, 1035-1040. http://dx.doi.org/10.1094/PD-89-1035
Solla A, Aguín O, Cubera E, Sampedro L, Mansilla J, Zas R. 2011. Survival time analysis of Pinus pinaster inoculated with Armillaria ostoyae: genetic variation and relevance of seed and root traits. Eur J Plant Pathol 130, 477-488. http://dx.doi.org/10.1007/s10658-011-9767-5
Vivas M, Zas R, Solla A. 2012. Screening of Maritime pine (Pinus pinaster) for resistance to Fusarium circinatum, the causal agent of Pitch Canker disease. Forestry 85, 185-192. http://dx.doi.org/10.1093/forestry/cpr055
Walters D, Heil M. 2007. Costs and trade-offs associated with induced resistance. Physiol Mol Plant Pathol 71, 3-17. http://dx.doi.org/10.1016/j.pmpp.2007.09.008
Wainhouse D, Staley JT, Jinks R, Morgan G. 2009. Growth and defence in young pine and spruce and the expression of resistance to a stem-feeding weevil. Oecologia 158, 641-650. http://dx.doi.org/10.1007/s00442-008-1173-0 PMid:18975014
Zeneli G, Krokene P, Christiansen E, Krekling T, Gershenzon J. 2006. Methyl jasmonate treatment of mature Norway spruce (Picea abies) trees increases the accumulation of terpenoid resin components and protects against infection by Ceratocystis polonica, a bark beetle-associated fungus. Tree Physiol 26, 977-988. http://dx.doi.org/10.1093/treephys/26.8.977 PMid:16651247
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